职 务：

无

职 称：

教授

评聘时间：

所在系：

环境科学系

专业：

环境微生物学

主要研究方向：

1. 废弃物与环境污染物微生物代谢；

2. 植物与土壤微生物组；

3. 碳氮循环微生物。

主讲课程：

高等微生物学

联系电话：

13520613975

电子邮箱：

dongfeihan@hotmail.com

个人简介：

专业环境微生物学；最全菠菜论坛硕士生导师；中国农业科学院农业环境与可持续发展研究所博士生导师、“青年英才计划”；德国马克思普朗克陆地微生物学研究所博士后。在中国、德国、韩国顶级科研机构长期从事微生物代谢与酶工程、甲烷代谢微生物、植物-微生物互作等研究，关于全新自给自足反式茴香烯加氧酶、甲烷氧化菌的环境适应性进化、植物微生物组功能等研究成果，在领域顶级期刊发表论文20余篇；主持参与国家自然科学基金，德国DFG基金、马尔堡大学合成微生物学计划(SYNMIKRO)、韩国农业振兴厅、韩国NRF等多项国家及国际项目；主持制定农业行业标准2项；发明专利4项。

学习经历：

2013-2018，德国马克思普朗克陆地微生物学研究所，博士后

2008-2013，韩国光州科学技术院，环境微生物学，博士

2006-2008，中国科学院遗传与发育生物学研究所，博士研究生

2003-2006，中国农业科学院作物科学研究所，生物化学与分子生物学，硕士

个人荣誉：

获得中国农业科学院“青年英才计划”、德国马普学会博士后奖学金、韩国光州科学技术院最佳博士论文奖、韩国原子能研究院经理奖、BK21奖学金、国家科技进步二等奖、《中国环境科学》年度优秀审稿人等奖励与称号。

社会兼职：

现为中国绿色食品协会专家委员会专家委员、全国研究生教育评估监测专家库专家、黑龙江省食品安全委员会专家库专家、中国农业科学院研究生院博士生导师、河北工程大学校外研究生导师、国际热带农业中心微生物协作网络(CIAT-CMBP)合作专家；Frontiers in Microbiology、Frontiers in Fungal Biology、Korean Journal of Environmental Agriculture、iMeta等国际期刊编委。

论文论著：

1. Yanshuo Pan, Surina Borjigin, Ye Liu, Hongzhe Wang, Yanan Wang, Yingchun Wu, Guangfei Hao, Daozhi Gong, Qingwen Yang, Zengchao Geng, Yuzhong Li,Dongfei Han^†. 2022. Role of key-stone microbial taxa in algae amended soil for mediating nitrogen transformation.Science of Total Env.doi:10.1016/j.scitotenv.2022.153547

2. Xingjie Wu, Christopher Rensing,Dongfei Han, Ke-Qing Xiao, Werner Liesack, Yuexiu Dai, Zhixi Tang, Jingjing Peng, Zhenling Cui, Fusuo Zhang. 2022. Genome-resolved metagenomics reveals distinct phosphorous acquisition strategies between soil microbiomes.mSystems,7(1): e01107-21.

3. Wenbo Fu, Yanshuo Pan, Yuhua Shi, Jieyin Chen, Daozhi Gong, Yuzhong Li, Guangfei Hao,Dongfei Han^†. 2022. Root morphogenesis ofArabidopsis thalianatuned by plant growth promotingStreptomycesisolated from root-associated soil ofArtemisia annua.Frontiers Plant Sci.12: 802737.

4. Hao Cao, Xinyu Zhang, Shuangyan Wang, Jiading Liu,Dongfei Han, Baisuo Zhao, Haisheng Wang. 2021. Insights into mechanism of the naphthalene-enhanced biodegradation of phenanthrene byPseudomonassp. SL-6 based on omics analysis.Frontiers Microbiol.fmicb.2021.761216.

5. Yuhua Shi, Yanshuo Pan, Li Xiang, Zhihui Zhu,Wenbo Fu, Guangfei Hao, Zengchao Geng, Shilin Chen, Yuzhong Li^†,Dongfei Han^†. 2021. Assembly of rhizosphere microbial communities inArtemisia annua: recruitment of plant growth-promoting microorganisms and inter-kingdom interactions between bacteria and fungi.Plant and Soil.s11104-021-04829-9.

6. Yingchun Wu, Guangfei Hao, andDongfei Han^†. (2021). Sample preparation for bacterial transcriptome analysis.Bioprotocol, e2003650.

7. Jintong Zhao, Xiaoqing Liu,Dongfei Han^†, and Jian Tian^†. 2021. Screening method of functional microorganisms interacting with rice roots.Bioprotocol, e2003638.

8. Yanshuo Han, Jian Tian, Yuzhong Li, Hor-Gil Hur,Dongfei Han^†. 2020. Complete genome sequence of a plant-derived phenylpropanoid-degrading bacterium,Pseudomonas putidaJYR-1,Microbiol. Resour. Announc.9:e01152-19.

9. Ye Yao, Bo Fu,Dongfei Han, Yan Zhang, He Liu. 2020. Formate-dependent acetogenic utilization of glucose by the fecal acetogenClostridium bovifaecis.Appl. Environ. Microbiol.86(23):e01870-20.

10. Nan Wang, Feifei Guan, Xiang Lv,Dongfei Han, Ningfeng Wu, Xiaole Xia, Jian Tian. 2020. Enhancing secretion of polyethylene terephthalate hydrolase PETase inBacillus subtilisWB600 mediated by the SPamy signal peptide,Letters Appl. Microbiol.71(3):235-241

11. Dongfei Han, Svetlana N. Dedysh, Werner Liesack. 2018. Unusual genomic traits suggestMethylocystis bryophilaS285 to be well adapted for life in peatlands.Genome Biol. Evol.10(2): 623-628.

12. Dongfei Han, Hannes Link, Werner Liesack. 2017. Response ofMethylocystissp. strain SC2 to salt stress: physiology, global transcriptome, and amino acid profiles.Appl. Environ. Microbiol.83: e00866-17. (Spotlight selected by editor)

13. Yue Sun*, Li Ma*,Dongfei Han*,Lei Du, Fengxia Qi, Wei Zhang, Jingran Sun, Shan Huang, Eung-Soo Kim, Shengying Li. 2017.In vitroreconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins,J. Ind. Microbiol. Biotechnol.44: 161-166.

14. Xianliang Zheng, Bo Fang,Dongfei Han,Wenxia Yang, Feifei Qi, Hui Chen, Shengying Li. 2016. Improving the secretory expression of anα-galactosidase fromAspergillus nigerinPichia pastoris, PLoS ONE 11(8): e0161529.

15. Ruirui Feng, Bo Liang, Chuantao Hou,Dongfei Han, Qiaolin Lang, Lihui Han, Aihua Liu. 2016. Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell,Enzyme Microb. Technol.84: 78-85.

16. Jianxia Song*, Bo Liang*,Dongfei Han*, Xiangjiang Tang, Qiaolin Lang, Ruirui Feng, Lihui Han, Aihua Liu. 2015. Bacterial cell-surface displaying of thermo-tolerant glutamate dehydrogenase and its application in L-glutamate assay,Enzyme Microb. Technol.70: 72-78.

17. Hyunji Lee, Jiyoung Park, Chaewon Jung,Dongfei Han, Jiyoung Seo, Joong-Hoon Ahn, Youhoon Chong, Hor-Gil Hur. 2015. Enhancement of the catalytic activity of ferulic acid decarboxylase fromEnterobactersp. Px6-4 through random and site-directed mutagenesis,Appl. Microbiol. Biotechnol.99: 9473-9481.

18. Xiangjiang Tang, Tingting Zhang, Bo Liang,Dongfei Han, Lingxing Zeng, Cheng Zheng, Tie Li, Mingdeng Wei, Aihua Liu. 2014. Sensitive electrochemical microbial biosensor forp-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons,Biosens. Bioelectron.60: 137-142.

19. Dongfei Han,Michael J. Sadowsky, Hor-Gil Hur. 2013. Characterization of a self-sufficienttrans-anethole oxygenase by Pseudomonas putidaJYR-1.PLoS ONE8(9): e73350.

20. Dongfei Han, Ji-Young Ryu, Hyunji Lee, Hor-Gil Hur. 2013. Bacterial biotransformation of phenylpropanoid compounds for producing flavor and fragrance compounds.App. Biol. Chem.56:125-133.

21. Dongfei Han, Somwang Kurusarttra, Ji-Young Ryu, Robert A. Kanaly, Hor-Gil Hur. 2012. Production of natural fragrance aromatic acids by coexpressingtrans-anethole oxygenase andp-anisaldehyde dehydrogenase genes ofPseudomonas putidaJYR-1 inEscherichia coli. J. Agric. Food Chem.60: 11972-11979.

22. Dongfei Han, Ji-Young Ryu, Robert A. Kanaly Hor-Gil Hur. 2012. Isolation of a gene responsible for the oxidation oftrans-anethole topara-anisaldehyde byPseudomonas putidaJYR-1 and its expression inE. coli.Appl. Environ. Microbiol.78: 5238-5246.

专利：

韩东飞，王宏哲. 2022.一种具有降解聚乙烯功能的戈登氏菌及其应用,专利号202210029072.3

韩东飞，付文博. 2021.一种促进植物根部生长的放线菌及其应用,专利号202111234302.1

Hor-Gil Hur，Dongfei Han,2011,A New-typetrans-Anethole Oxygenase.Korean Patent No. 10-2011-002-367

承担项目：

2018-2023，“青年英才”计划启动经费，200万元，科技创新工程，中国农业科学院，主持

2020，农业环境标准制定，50万元，农业行业标准类项目，农业农村部，主持

2020-2022，农业微生物代谢调节剂产品规范，10万元，横向，主持

2020-2022，农业微生物菌剂产品规范，10万元，横向，主持

2014-2016，自给自足反式茴香烯加氧酶的催化机理研究，22万元，青年科学基金项目，31300663,国家自然科学基金，主持

2016-2020，Metabolic adaptations in response to perception of environmental changes, Deutsche Forschungs gemeinschaft (DFG)，参加

2012-2016，Producing and studying synthetic microbial communities to be compared to natural communities. LOEWE center for Synthetic Microbiology (SYNMIKRO)，参加

2010-2015，环境修复Smart BioNanofactory技术开发,韩国国家研究基金，参加

2006-2009，微生物转化植物源物质生成芳香化合物的代谢机理研究，韩国农村振兴厅，参加

2003-2006，农业野生植物保护与可持续利用，国家财政专项，参加

获奖成果：

2017.12，国家科技进步奖二等奖，中国野生稻种质资源保护与创新利用，主要完成人